› 1c77

X-ray diffraction
2.3Å resolution

STAPHYLOKINASE (SAK) DIMER

Released:
DOI: 10.2210/pdb1c77/pdb
PDB entry 1c77 coloured by chain, front view.
PDB entry 1c77 coloured by chain, side view.
PDB entry 1c77 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal structure of a staphylokinase: variant a model for reduced antigenicity.
Chen Y, Song G, Jiang F, Feng L, Zhang X, Ding Y, Bartlam M, Yang A, Ma X, Ye S, Liu Y, Tang H, Song H, Rao Z
Eur J Biochem 269 705-11 (2002)
PMID: 11856331

Function and Biology Details

Reaction catalysed: 
Cleavage of insulin B chain with specificity similar to that of thermolysin, preferring hydrophobic P1' residue. Activates the glutamyl endopeptidase (EC 3.4.21.19) of Staphylococcus aureus.
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159507 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Staphylokinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 136 amino acids
Theoretical weight: 15.49 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P68802 (Residues: 28-163; Coverage: 100%)
Gene name: sak
Sequence domains: Staphylokinase/Streptokinase family
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 43.87Å b: 59.26Å c: 102.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.198 0.267
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Novel self-cleavage activity of Staphylokinase fusion proteins: An interesting finding and its possible applications.
Prasad et al. (2010)
3 more

2 mentions without citation

Chemical shift-based methods in NMR structure determination.
Nerli et al. (2018)
1 more