› 1bhc

X-ray diffraction
2.7Å resolution

BOVINE PANCREATIC TRYPSIN INHIBITOR CRYSTALLIZED FROM THIOCYANATE

Released:
DOI: 10.2210/pdb1bhc/pdb
PDB entry 1bhc coloured by chain, front view.
PDB entry 1bhc coloured by chain, side view.
PDB entry 1bhc coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The decameric structure of bovine pancreatic trypsin inhibitor (BPTI) crystallized from thiocyanate at 2.7 A resolution.
Hamiaux C, Prangé T, Riès-Kautt M, Ducruix A, Lafont S, Astier JP, Veesler S
Acta Crystallogr D Biol Crystallogr 55 103-13 (1999)
PMID: 10089400

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133761 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pancreatic trypsin inhibitor Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 58 amino acids
Theoretical weight: 6.53 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00974 (Residues: 36-93; Coverage: 73%)
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

1 bound ligand:
Ligand SCN 10 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P21
Unit cell:
a: 71.56Å b: 73.83Å c: 64.47Å
α: 90° β: 93.91° γ: 90°
R-values:
R R work R free
0.201 0.201 0.265

Quick links

Citations

1 review citation

Ion hydration: Implications for cellular function, polyelectrolytes, and protein crystallization.
Collins KD. (2006)
9 other articles

3 mentions without citation

Bluues: a program for the analysis of the electrostatic properties of proteins based on generalized Born radii.
Fogolari et al. (2012)
2 more