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Solution NMR

NON-SPECIFIC PROTEIN-DNA INTERACTIONS IN THE SSO7D-DNA COMPLEX, NMR, 1 STRUCTURE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA-binding protein 7d Chains: C, D
Molecule details ›
Chains: C, D
Length: 63 amino acids
Theoretical weight: 7.16 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39476 (Residues: 2-64; Coverage: 98%)
Gene names: SSO10610, sso7d, sso7d-1
Sequence domains: 7kD DNA-binding domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
DNA (5'-D(*CP*TP*AP*GP*CP*GP*CP*GP*CP*TP*AP*G)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Saccharolobus solfataricus
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 13%
Refinement method: simulated annealing
Chemical shifts: BMR5909   BMR5910  
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided