PDBe 1bbr

X-ray diffraction
2.3Å resolution

THE STRUCTURE OF RESIDUES 7-16 OF THE A ALPHA CHAIN OF HUMAN FIBRINOGEN BOUND TO BOVINE THROMBIN AT 2.3 ANGSTROMS RESOLUTION

Released:

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero trimer
hetero hexamer
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Thrombin light chain Chains: J, L, M
Molecule details ›
Chains: J, L, M
Length: 49 amino acids
Theoretical weight: 5.74 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P00735 (Residues: 318-366; Coverage: 8%)
Gene name: F2
Sequence domains: Thrombin light chain
Thrombin heavy chain Chain: H
Molecule details ›
Chain: H
Length: 150 amino acids
Theoretical weight: 17.53 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P00735 (Residues: 367-516; Coverage: 25%)
Gene name: F2
Structure domains: Trypsin-like serine proteases
Thrombin heavy chain Chain: E
Molecule details ›
Chain: E
Length: 109 amino acids
Theoretical weight: 12.27 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P00735 (Residues: 517-625; Coverage: 18%)
Gene name: F2
Structure domains: Trypsin-like serine proteases
Fibrinopeptide A Chains: F, G, I
Molecule details ›
Chains: F, G, I
Length: 11 amino acids
Theoretical weight: 1.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P02671 (Residues: 26-35; Coverage: 1%)
Gene name: FGA
Thrombin heavy chain Chains: K, N
Molecule details ›
Chains: K, N
Length: 259 amino acids
Theoretical weight: 29.77 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P00735 (Residues: 367-625; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 83.02Å b: 89.41Å c: 99.3Å
α: 90° β: 106.64° γ: 90°
R-values:
R R work R free
0.167 not available not available
Expression system: Not provided