› 1ba1

X-ray diffraction
1.7Å resolution

HEAT-SHOCK COGNATE 70KD PROTEIN 44KD ATPASE N-TERMINAL MUTANT WITH CYS 17 REPLACED BY LYS

Released:
DOI: 10.2210/pdb1ba1/pdb
PDB entry 1ba1 coloured by chain, front view.
PDB entry 1ba1 coloured by chain, side view.
PDB entry 1ba1 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural replacement of active site monovalent cations by the epsilon-amino group of lysine in the ATPase fragment of bovine Hsc70.
Wilbanks SM, McKay DB
Biochemistry 37 7456-62 (1998)
PMID: 9585559

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148503 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat shock cognate 71 kDa protein Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 42.54 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19120 (Residues: 1-386; Coverage: 59%)
Gene names: HSC70, HSPA8
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand NA 1 x NA
Ligand CL 2 x CL
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 143.8Å b: 64.1Å c: 46.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.244
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Thrombin.
Di Cera E. (2008)
1 more

7 mentions without citation

F-actin-like filaments formed by plasmid segregation protein ParM.
van den Ent et al. (2002)
6 more