PDBe 1avp

X-ray diffraction
2.6Å resolution

STRUCTURE OF HUMAN ADENOVIRUS 2 PROTEINASE WITH ITS 11 AMINO ACID COFACTOR

Released:

Function and Biology Details

Reaction catalysed:
Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-|-Xaa- and -Yaa-Xaa-Gly-Xaa-|-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid).
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protease Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.11 KDa
Source organism: Human adenovirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03252 (Residues: 1-204; Coverage: 100%)
Gene name: L3
Sequence domains: Adenovirus endoprotease
Structure domains: Adenoviral Proteinase; Chain A
Protease cofactor Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.35 KDa
Source organism: Human adenovirus 2
Expression system: Not provided
UniProt:
  • Canonical: P03274 (Residues: 240-250; Coverage: 4%)
Gene name: L3

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P61
Unit cell:
a: 114.3Å b: 114.3Å c: 50Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.184 0.233
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided