PDBe 1aro

X-ray diffraction
2.8Å resolution

T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Endolysin Chain: L
Molecule details ›
Chain: L
Length: 151 amino acids
Theoretical weight: 17.01 KDa
Source organism: Enterobacteria phage T7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00806 (Residues: 1-151; Coverage: 100%)
Gene name: 3.5
Sequence domains: N-acetylmuramoyl-L-alanine amidase
Structure domains: Peptidoglycan recognition protein-like
T7 RNA polymerase Chain: P
Molecule details ›
Chain: P
Length: 883 amino acids
Theoretical weight: 98.94 KDa
Source organism: Enterobacteria phage T7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00573 (Residues: 1-883; Coverage: 100%)
Gene name: 1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: C2
Unit cell:
a: 273.385Å b: 95.612Å c: 63.582Å
α: 90° β: 101.4° γ: 90°
R-values:
R R work R free
0.262 0.262 0.309
Expression system: Escherichia coli BL21