1a36

X-ray diffraction
2.8Å resolution

TOPOISOMERASE I/DNA COMPLEX

Released:
Source organism: Homo sapiens
Primary publication:
A model for the mechanism of human topoisomerase I.
Science 279 1534-41 (1998)
PMID: 9488652

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA topoisomerase 1 Chain: A
Molecule details ›
Chain: A
Length: 591 amino acids
Theoretical weight: 70.02 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P11387 (Residues: 175-765; Coverage: 77%)
Gene name: TOP1
Sequence domains:
Structure domains:
DNA (5'-D(*AP*AP*AP*AP*AP*GP*AP*CP*TP*TP*AP*GP*AP*AP*AP*AP*A P*TP*TP*TP*TP*T)- 3') Chain: B
Molecule details ›
Chain: B
Length: 22 nucleotides
Theoretical weight: 6.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*AP*AP*AP*AP*AP*TP*TP*TP*TP*TP*CP*TP*AP*AP*GP*TP*C P*TP*TP*TP*TP*T)- 3') Chain: C
Molecule details ›
Chain: C
Length: 22 nucleotides
Theoretical weight: 6.71 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P21
Unit cell:
a: 56.5Å b: 118.4Å c: 71.5Å
α: 90° β: 101.2° γ: 90°
R-values:
R R work R free
0.224 0.224 0.312
Expression systems:
  • Spodoptera frugiperda
  • Not provided