› 191l

X-ray diffraction
1.95Å resolution

A HELIX INITIATION SIGNAL IN T4 LYSOZYME IDENTIFIED BY POLYALANINE MUTAGENESIS

Released:
DOI: 10.2210/pdb191l/pdb
PDB entry 191l coloured by chain, front view.
PDB entry 191l coloured by chain, side view.
PDB entry 191l coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
A helix initiation signal in T4 lysozyme identified by polyalanine mutagenesis.
Zhang XJ, Baase WA, Matthews BW
Biophys Chem 101-102 43-56 (2002)
PMID: 12487988

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.42 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 1-164; Coverage: 100%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand HED 1 x HED
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3221
Unit cell:
a: 61Å b: 61Å c: 97.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 not available not available
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structural origin of weakly ordered nitroxide motion in spin-labeled proteins.
Fleissner et al. (2009)

3 mentions without citation

Genomic and proteomic analysis of invertebrate iridovirus type 9.
Wong et al. (2011)
2 more