› 146l

X-ray diffraction
1.85Å resolution

ROLE OF BACKBONE FLEXIBILITY IN THE ACCOMMODATION OF VARIANTS THAT REPACK THE CORE OF T4 LYSOZYME

Released:
DOI: 10.2210/pdb146l/pdb
PDB entry 146l coloured by chain, front view.
PDB entry 146l coloured by chain, side view.
PDB entry 146l coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
The role of backbone flexibility in the accommodation of variants that repack the core of T4 lysozyme.
Baldwin EP, Hajiseyedjavadi O, Baase WA, Matthews BW
Science 262 1715-8 (1993)
PMID: 8259514

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.76 KDa
Source organism: Escherichia virus T4
Expression system: Not provided
UniProt:
  • Canonical: P00720 (Residues: 1-164; Coverage: 100%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand BME 2 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3221
Unit cell:
a: 61.1Å b: 61.1Å c: 97Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 not available not available
Expression system: Not provided

Quick links

Citations

20 review citations

Principles of protein folding--a perspective from simple exact models.
Dill et al. (1995)
19 more

2 mentions without citation

Six Rossmannoid folds, including the Class I aminoacyl-tRNA synthetases, share a partial core with the anti-codon-binding domain of a Class II aminoacyl-tRNA synthetase.
Cammer et al. (2010)
1 more