› 108m

X-ray diffraction
2.67Å resolution

SPERM WHALE MYOGLOBIN V68F N-BUTYL ISOCYANIDE AT PH 7.0

Released:
DOI: 10.2210/pdb108m/pdb
PDB entry 108m coloured by chain, front view.
PDB entry 108m coloured by chain, side view.
PDB entry 108m coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
Correlations between Bound N-Alkyl Isocyanide Orientations and Pathways for Ligand Binding in Recombinant Myoglobins
Smith RD
Thesis, Rice (1999)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.41 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli
UniProt:
  • Canonical: P02185 (Residues: 1-154; Coverage: 100%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand HEM 1 x HEM
Ligand NBN 1 x NBN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P6
Unit cell:
a: 91.622Å b: 91.622Å c: 45.984Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.124 0.124 0.226
Expression system: Escherichia coli

Quick links

Citations

2 mentions without citation

A hotspot of inactivation: The A22S and V108M polymorphisms individually destabilize the active site structure of catechol O-methyltransferase.
Rutherford et al. (2009)
1 more