EMD-9931 Experiments and Validation

Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1 state class1)

Single particle reconstruction
Overview of EMD-9931
Sample name: ATP8A1-CDC50a
Organism: Homo sapiens
Fitted atomic model: 6k7g

Map parameters

Recommended contour level: 0.015 (author)
Number of grid points: 260 × 260 × 260
Voxel size: 0.83 × 0.83 × 0.83 Å
Minimum density: -0.085
Maximum density: 0.141
Average density: 0.00
Standard deviation: 0.004

Sample information

Sample name: ATP8A1-CDC50a
Ligands: 2-acetamido-2-deoxy-beta-D-glucopyranose, CHOLESTEROL HEMISUCCINATE
Proteins: ATP8A1-CDC50a, Phospholipid-transporting ATPase, Cell cycle control protein 50A

Validation information

Experimental information

 

Image processing

Number of particles: 139434

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 64.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Vitrification
Cryogen: ETHANE