EMD-9843

Single-particle
7.3 Å
EMD-9843
3D View Gallery
Deposition: 07/03/2019
Map released: 15/05/2019
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-9843
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6jm9 summary report (pdf.gz) Map and 6jm9 FULL report (pdf.gz)
EMICSS annotations (xml)

cryo-EM structure of DOT1L bound to unmodified nucleosome

EMD-9843

Single-particle
7.3 Å
EMD-9843
3D View Gallery
Deposition: 07/03/2019
Map released: 15/05/2019
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN
Illumination mode: OTHER
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 37.28 e/Å2
Image processing [1]
Final reconstruction
Resolution: 7.3 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 21229
Startup model [1]
Type: OTHER
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Structure of catalytic domain of DOT1L bound to core nucleosome
⬡ Geometry
X Y Z
Dimensions 200 200 200
Origin 0 0 0
Spacing 200 200 200
Voxel size 1.06 Å 1.06 Å 1.06 Å
Contour list
Primary Level Source
True 0.021 AUTHOR