EMD-9791 Experiments and Validation

Cryo-EM structure of human lysosomal cobalamin exporter ABCD4

Single particle reconstruction
Overview of EMD-9791
Sample name: Human ABCD4
Organism: Homo sapiens
Fitted atomic model: 6jbj

Map parameters

Recommended contour level: 0.12 (author)
Number of grid points: 200 × 200 × 200
Voxel size: 1.04 × 1.04 × 1.04 Å
Minimum density: -0.094
Maximum density: 0.168
Average density: 0.00
Standard deviation: 0.006

Sample information

Sample name: Human ABCD4
Ligand: ADENOSINE-5'-TRIPHOSPHATE
Proteins: Human ABCD4, ATP-binding cassette sub-family D member 4

Validation information

Experimental information

 

Image processing

Number of particles: 276557

Fitting

Overall B value: 199.0

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 60.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 4.50
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100