EMD-9593
Trypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein and ACE2 complex, ACE2-bound conformation 2
EMD-9593
Single-particle4.2 Å
Deposition: 26/07/2018
Map released: 08/08/2018
Last modified: 06/11/2019
Buffer
pH: 5.8
Vitrification
Cryogen name: ETHANE
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording
[1]
Detector model:
GATAN K2 SUMMIT (4k x 4k)
Average exposure time: 8.0 s
Average electron dose per image: 50.0 e/Å2
Average exposure time: 8.0 s
Average electron dose per image: 50.0 e/Å2
Final
reconstruction
Resolution: 4.2
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 129462
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 129462
⌯ Applied Symmetry
Point group:
C1
Software
[1]
Name | Version | Details |
---|---|---|
RELION | 1.4 | - |
⦨ Initial angle
assignment
Type:
COMMON LINE
⦩ Final angle assignment
Type:
PROJECTION MATCHING
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 288 | 288 | 288 |
Origin | 0 | 0 | 0 |
Spacing | 288 | 288 | 288 |
Voxel size | 1.32 Å | 1.32 Å | 1.32 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 8.0 | AUTHOR |