EMD-9187

Single-particle
3.54 Å
EMD-9187
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Deposition: 05/10/2018
Map released: 06/11/2019
Last modified: 29/07/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-9187
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6mp6 summary report (pdf.gz) Map and 6mp6 FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of the human neutral amino acid transporter ASCT2

EMD-9187

Single-particle
3.54 Å
EMD-9187
3D View Gallery
Deposition: 05/10/2018
Map released: 06/11/2019
Last modified: 29/07/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 2 mg/mL
Buffer
pH: 7.5
Grid
Mesh: 200
Model: Quantifoil, UltrAuFoil, R1.2/1.3
Material: GOLD
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 100.0 µm
Nominal CS: 2.7 mm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Alignment procedure: COMA FREE
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Average electron dose per image: 1.06 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.54 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 165067
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
CisTEM - -
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION - -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
CisTEM - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Neutral amino acid transporter
⬡ Geometry
X Y Z
Dimensions 224 224 224
Origin 0 0 0
Spacing 224 224 224
Voxel size 1.086 Å 1.086 Å 1.086 Å
Contour list
Primary Level Source
True 2.0 AUTHOR