EMD-9119 Experiments and Validation

Structure of human TRPV3 in the presence of 2-APB in C4 symmetry

Single particle reconstruction
Overview of EMD-9119
Sample name: Human TRPV3 in the presence of 2-APB
Organism: Homo sapiens
Fitted atomic model: 6mhv
Related EM entries by publication: EMD-9115, EMD-9117, EMD-9120, EMD-9121

Map parameters

Recommended contour level: 0.07 (author)
Number of grid points: 288 × 288 × 288
Voxel size: 1.15 × 1.15 × 1.15 Å
Minimum density: -0.296
Maximum density: 0.527
Average density: 0.001
Standard deviation: 0.011

Sample information

Sample name: Human TRPV3 in the presence of 2-APB
Proteins: Human TRPV3 in the presence of 2-APB, Transient receptor potential cation channel subfamily V member 3

Validation information

Experimental information

 

Image processing

Applied symmetry: C4
Software: RELION
Number of particles: 141832
Reconstruction protocol: BACK PROJECTION

Fitting

Overall B value: 145.0
Refinement space: REAL

Imaging

Session
Microscope model: FEI TALOS ARCTICA
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 60.0
Nominal CS (mm): 2.70
Nominal magnification: 36000.0
Defocus max (nm): 2,000.00
Defocus min (nm): 1,200.00
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Details: Alignment performed as described in Herzik, Wu, Lander, Nat Methods 2017 (PMID: 28991891)
Acquisition
Number of images: 1908
Sampling interval (μm): 5.0

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 0.50
Buffer: Buffer contained 1 mM 2-APB
Specimen support: Gatan Solarus
Vitrification
Apparatus: HOMEMADE PLUNGER
Cryogen: ETHANE
Humidity: 90
Details: Specimen was manually blotted for 4 seconds immediately prior to plunge-freezing in liquid ethane cooled by liquid nitrogen..