EMD-8977 Function and Biology

Cryo-EM structure at 3.2 A resolution of HIV-1 fusion peptide-directed antibody, A12V163-b.01, elicited by vaccination of Rhesus macaques, in complex with stabilized HIV-1 Env BG505 DS-SOSIP, which was also bound to antibodies VRC03 and PGT122

Single particle reconstruction
3.18Å resolution
Overview of EMD-8977
Sample name: Quaternary complex of HIV-1 Env BG505 DS-SOSIP with antibodies A12V163-b.01, VRC03 and PGT122.
Number of unique components: 14

Quaternary complex of HIV-1 Env BG505 DS-SOSIP with antibodies A12V163-b.01, VRC03 and PGT122.

Protein type
Name: Quaternary complex of HIV-1 Env BG505 DS-SOSIP with antibodies A12V163-b.01, VRC03 and PGT122.
Theoretical weight (MDa): 0.63

HIV-1 Env BG505 DS-SOSIP gp120 [Human immunodeficiency virus 1]

Protein type
Name: HIV-1 Env BG505 DS-SOSIP gp120
Expression system
Expression system: Homo sapiens

HIV-1 Env BG505 DS-SOSIP gp41 [Human immunodeficiency virus 1]

Protein type
Name: HIV-1 Env BG505 DS-SOSIP gp41
Expression system
Expression system: Homo sapiens

A12V163-b.01 [Macaca mulatta]

Protein type
Name: A12V163-b.01
Expression system
Expression system: Homo sapiens

VRC03 [Homo sapiens]

Protein type
Name: VRC03
Expression system
Expression system: Homo sapiens

PGT122 [Homo sapiens]

Protein type
Name: PGT122
Expression system
Expression system: Homo sapiens

HIV-1 Env BG505 DS-SOSIP gp120 [Human immunodeficiency virus 1]

Protein type
Name: HIV-1 Env BG505 DS-SOSIP gp120
Expression system
Expression system: Homo sapiens

HIV-1 Env BG505 DS-SOSIP gp41 [Human immunodeficiency virus 1]

Protein type
Name: HIV-1 Env BG505 DS-SOSIP gp41
Expression system
Expression system: Homo sapiens

A12V163-b.01 Heavy chain [Macaca mulatta]

Protein type
Name: A12V163-b.01 Heavy chain
Expression system
Expression system: Homo sapiens

A12V163-b.01 Light chain [Macaca mulatta]

Protein type
Name: A12V163-b.01 Light chain
Expression system
Expression system: Homo sapiens

VRC03 heavy chain [Homo sapiens]

Protein type
Name: VRC03 heavy chain
Expression system
Expression system: Homo sapiens

VRC03 light chain [Homo sapiens]

Protein type
Name: VRC03 light chain
Expression system
Expression system: Homo sapiens

PGT122 heavy chain [Homo sapiens]

Protein type
Name: PGT122 heavy chain
Expression system
Expression system: Homo sapiens

PGT122 light chain [Homo sapiens]

Protein type
Name: PGT122 light chain
Expression system
Expression system: Homo sapiens