EMD-8921

Single-particle
4.24 Å
EMD-8921
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Deposition: 25/06/2018
Map released: 05/09/2018
Last modified: 18/12/2019
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-8921
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6dvz summary report (pdf.gz) Map and 6dvz FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of mouse TRPV3-Y564A in complex with 2-Aminoethoxydiphenyl borate (2-APB)

EMD-8921

Single-particle
4.24 Å
EMD-8921
3D View Gallery
Deposition: 25/06/2018
Map released: 05/09/2018
Last modified: 18/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 4.0 mg/mL
Buffer
pH: 8.0
Buffer components [3]:
Name Formula Concentration ChEBI
Sodium chloride NaCl 150.0 mM
TRIS TRIS 20.0 mM
GDN GDN 0.08 mM
Grid
Material: GOLD
Support Film [1]
Material Topology Thickness
CARBON HOLEY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.24 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 28075
Software [1]
Name Version Details
RELION - -
Startup model [1]
Type: EMDB MAP
EMDB model: EMD-8919
⦨ Initial angle assignment
Type: ANGULAR RECONSTITUTION
⦩ Final angle assignment
Type: ANGULAR RECONSTITUTION
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 220 220 220
Origin 0 0 0
Spacing 220 220 220
Voxel size 1.06 Å 1.06 Å 1.06 Å
Contour list
Primary Level Source
True 0.057 AUTHOR