EMD-8702 Experiments and Validation

Structure of a mechanotransduction ion channel Drosophila NOMPC in nanodisc

Single particle reconstruction
Overview of EMD-8702
Sample name: Tetrameric channel
Organism: Drosophila melanogaster
Fitted atomic model: 5vkq

Map parameters

Recommended contour level: 10.00 (author)
Number of grid points: 400 × 400 × 400
Voxel size: 1.22 × 1.22 × 1.22 Å
Minimum density: -27.667
Maximum density: 60.021
Average density: -0.243
Standard deviation: 1.00

Sample information

Sample name: Tetrameric channel
Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSHOCHOLINE
Proteins: Tetrameric channel, No mechanoreceptor potential C isoform L

Validation information

Experimental information

 

Image processing

Applied symmetry: C4
Software: RELION
Number of particles: 175314

Fitting

Overall B value: 125.7
Refinement space: REAL

Imaging

Session
Microscope model: FEI POLARA 300
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 54.0
Nominal CS (mm): 2.10
Nominal magnification: 31000.0
Defocus max (nm): -3,000.00
Defocus min (nm): -1,500.00
Holder model: GATAN HELIUM
Acquisition
Number of images: 1873
Sampling interval (μm): 5.0

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 3.40
Vitrification
Apparatus: GATAN CRYOPLUNGE 3
Cryogen: ETHANE
Humidity: 92
Details: 2.5 sec blot, -1 offset, whatman #1 filter paper on front side, plastic on back side.