EMD-8560 Experiments and Validation

Cryo-EM structure of bovine multidrug resistance protein 1 (MRP1) bound to leukotriene C4

Single particle reconstruction
Overview of EMD-8560
Sample name: bovine multidrug resistance protein 1 (MRP1) complexed with leukotriene C4
Organism: Bos taurus
Fitted atomic model: 5uja
Related EM entry by publication: EMD-8559

Map parameters

Recommended contour level: 1.60 (author)
Number of grid points: 384 × 384 × 384
Voxel size: 0.38 × 0.26 × 0.28 Å
Minimum density: -4.154
Maximum density: 5.214
Average density: 0.212
Standard deviation: 0.618

Sample information

Sample name: bovine multidrug resistance protein 1 (MRP1) complexed with leukotriene C4
Ligand: (5~{S},6~{R},7~{E},9~{E},11~{Z},14~{Z})-6-[(2~{R})-2-[[(4~{S})-4-azanyl-5-oxidanyl-5-oxidanylidene-pentanoyl]amino]-3-(2-hydroxy-2-oxoethylamino)-3-oxidanylidene-propyl]sulfanyl-5-oxidanyl-icosa-7,9,11,14-tetraenoic acid
Proteins: bovine multidrug resistance protein 1 (MRP1) complexed with leukotriene C4, bovine multidrug resistance protein 1 (MRP1),Multidrug resistance-associated protein 1

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: FREALIGN
Number of particles: 203732

Fitting

Refinement space: RECIPROCAL

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 84.0
Nominal CS (mm): 2.70
Nominal magnification: 37000.0
Defocus max (nm): 2,400.00
Defocus min (nm): 700.00
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Acquisition
Number of images: 2302

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 4.65
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100