EMD-8516

Single-particle
3.87 Å
EMD-8516
3D View Gallery
Deposition: 10/12/2016
Map released: 21/12/2016
Last modified: 12/09/2018
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-8516
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5uak summary report (pdf.gz) Map and 5uak FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of human cystic fibrosis transmembrane conductance regulator (CFTR)

EMD-8516

Single-particle
3.87 Å
EMD-8516
3D View Gallery
Deposition: 10/12/2016
Map released: 21/12/2016
Last modified: 12/09/2018
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 5 mg/mL
Buffer
pH: 7.5
Grid
Mesh: 400
Model: Quantifoil R1.2/1.3
Material: GOLD
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Support Film [1]
Material Topology Thickness
CARBON HOLEY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 298 K
Instrument: FEI VITROBOT MARK I
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 0.8 µm - 2.5 µm
Calibrated magnification: 61200.0
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Dimensions: 3710 pixel x 3838 pixel
Frames per image: 1-50
Number of grids: 1
Number of real images: 3642
Average exposure time: 0.14 s
Average electron dose per image: 1.68 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.87 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of classed used: 1
Number of images used: 415915
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
FREALIGN - -
Startup model [1]
Type: EMDB MAP
EMDB model: EMD-8461
⦨ Initial angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
FREALIGN - -
⦩ Final angle assignment
Type: ANGULAR RECONSTITUTION
Software [1]
Name Version Details
FREALIGN - -
Particle selection [1]
Selected Ref. model Method Software Details
430000 - - - -
CTF correction
Software [1]
Name Version Details
CTFFIND4 4.0.17 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Human cystic fibrosis transmembrane conductance regulator, B sharpened map
⬡ Geometry
X Y Z
Dimensions 384 384 384
Origin 0 0 0
Spacing 384 384 384
Voxel size 0.817 Å 0.817 Å 0.817 Å
Contour list
Primary Level Source
True 0.781 AUTHOR