EMD-8331

Single-particle
3.4 Å
EMD-8331
3D View Gallery
Deposition: 30/08/2016
Map released: 14/09/2016
Last modified: 29/07/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-8331
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5szs summary report (pdf.gz) Map and 5szs FULL report (pdf.gz)
EMICSS annotations (xml)

Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy

EMD-8331

Single-particle
3.4 Å
EMD-8331
3D View Gallery
Deposition: 30/08/2016
Map released: 14/09/2016
Last modified: 29/07/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 1 mg/mL
Buffer
pH: 7.5
Buffer components [2]:
Name Formula Concentration ChEBI
Tris-HCl - 20.0 mM -
NaCl - 100.0 mM -
Grid
Mesh: 400
Model: Protochips C-flat 1.2/1.3
Material: COPPER
Details: 20 mA
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Sampling interval: 5.0 µm
Frames per image: 1-50
Average exposure time: 10.0 s
Average electron dose per image: 48.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.4 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 79667
Algorithm: FOURIER SPACE
⌯ Applied Symmetry
Point group: C3
Software [1]
Name Version Details
RELION - -
Startup model [1]
Type: OTHER
Details:Common-lines generated model for the MHV spike glycoprotein
⦨ Initial angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION - -
⦩ Final angle assignment
Type: PROJECTION MATCHING
Software [1]
Name Version Details
RELION - -
Particle selection [1]
Selected Ref. model Method Software Details
474000 - - - -
Final 3D classification
Software [1]
Name Version Details
RELION - -
CTF correction
Software [1]
Name Version Details
GCTF/Relion - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Coronavirus spike protein
⬡ Geometry
X Y Z
Dimensions 320 320 320
Origin 0 0 0
Spacing 320 320 320
Voxel size 1.36 Å 1.36 Å 1.36 Å
Contour list
Primary Level Source
True 0.055 AUTHOR