EMD-8289 Experiments and Validation

GluK2EM with 2S,4R-4-methylglutamate

Single particle reconstruction
Overview of EMD-8289
Sample name: GluK2EM with 2S,4R-4-methylglutamate
Organism: Rattus norvegicus
Fitted atomic model: 5kuf
Related EM entry by publication: EMD-8290

Map parameters

Recommended contour level: 0.037 (author)
Number of grid points: 300 × 300 × 300
Voxel size: 1.32 × 1.32 × 1.32 Å
Minimum density: -0.08
Maximum density: 0.161
Average density: -0.00
Standard deviation: 0.007

Sample information

Sample name: GluK2EM with 2S,4R-4-methylglutamate
Ligand: 2S,4R-4-METHYLGLUTAMATE
Proteins: GluK2EM with 2S,4R-4-methylglutamate, Glutamate receptor ionotropic, kainate 2

Validation information

Experimental information

 

Image processing

Applied symmetry: C2
Software: RELION
Number of particles: 62244

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 45.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 4.20
Vitrification
Cryogen: ETHANE