EMD-8136

Single-particle
3.9 Å
EMD-8136
3D View Gallery
Deposition: 02/05/2016
Map released: 18/05/2016
Last modified: 06/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-8136
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5iyb summary report (pdf.gz) Map and 5iyb FULL report (pdf.gz)
EMICSS annotations (xml)

Human core-PIC in the open state

EMD-8136

Single-particle
3.9 Å
EMD-8136
3D View Gallery
Deposition: 02/05/2016
Map released: 18/05/2016
Last modified: 06/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.3 mg/mL
Buffer
pH: 7.9
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Details: Blot for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK IV)..
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 2.0 µm - 4.0 µm
Nominal magnification: 27500.0
Specimen holder model: GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 42.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.9 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 79849
Software [1]
Name Version Details
RELION 1.4beta -
Startup model [1]
Type: OTHER
Details:Negative-stain reconstruction of a similar complex, low-pass filtered to 60 Angstrom resolution
⦨ Initial angle assignment
Type: PROJECTION MATCHING
⦩ Final angle assignment
Type: PROJECTION MATCHING
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: None
⬡ Geometry
X Y Z
Dimensions 384 384 384
Origin 0 0 0
Spacing 384 384 384
Voxel size 1.31 Å 1.31 Å 1.31 Å
Contour list
Primary Level Source
True 0.01 AUTHOR