EMD-8136 Function and Biology

Human core-PIC in the open state

Single particle reconstruction
3.9Å resolution
Overview of EMD-8136
Sample name: Human core-PIC in the open state
Number of unique components: 29

Human core-PIC in the open state [Homo sapiens]

Protein type
Name: Human core-PIC in the open state
Theoretical weight (MDa): 0.9

RNA polymerase II [Homo sapiens]

Protein type
Name: RNA polymerase II
Theoretical weight (MDa): 0.52

General transcription factors [Homo sapiens]

Protein type
Name: General transcription factors
Theoretical weight (MDa): 0.33

Super core promoter [Homo sapiens]

Protein type
Name: Super core promoter
Theoretical weight (MDa): 0.06

DNA-directed RNA polymerase II subunit RPB1 [Human]

Protein type
Name: DNA-directed RNA polymerase II subunit RPB1
Theoretical weight (MDa): 0.217
Number of copies: 1

DNA-directed RNA polymerase II subunit RPB2 [Human]

Protein type
Name: DNA-directed RNA polymerase II subunit RPB2
Theoretical weight (MDa): 0.134
Number of copies: 1

DNA-directed RNA polymerase II subunit RPB3 [Human]

Protein type
Name: DNA-directed RNA polymerase II subunit RPB3
Theoretical weight (MDa): 0.031
Number of copies: 1

DNA-directed RNA polymerase II subunit RPB4 [Human]

Protein type
Name: DNA-directed RNA polymerase II subunit RPB4
Theoretical weight (MDa): 0.016
Number of copies: 1

DNA-directed RNA polymerase II subunit RPB5 [Human]

Protein type
Name: DNA-directed RNA polymerase II subunit RPB5
Theoretical weight (MDa): 0.025
Number of copies: 1

DNA-directed RNA polymerase II subunit RPB6 [Human]

Protein type
Name: DNA-directed RNA polymerase II subunit RPB6
Theoretical weight (MDa): 0.014
Number of copies: 1

DNA-directed RNA polymerase II subunit RPB7 [Human]

Protein type
Name: DNA-directed RNA polymerase II subunit RPB7
Theoretical weight (MDa): 0.019
Number of copies: 1

DNA-directed RNA polymerase II subunit RPB8 [Human]

Protein type
Name: DNA-directed RNA polymerase II subunit RPB8
Theoretical weight (MDa): 0.017
Number of copies: 1

DNA-directed RNA polymerase II subunit RPB9 [Human]

Protein type
Name: DNA-directed RNA polymerase II subunit RPB9
Theoretical weight (MDa): 0.015
Number of copies: 1

DNA-directed RNA polymerase II subunit RPB10 [Human]

Protein type
Name: DNA-directed RNA polymerase II subunit RPB10
Theoretical weight (MDa): 0.008
Number of copies: 1

DNA-directed RNA polymerase II subunit RPB11-a [Human]

Protein type
Name: DNA-directed RNA polymerase II subunit RPB11-a
Theoretical weight (MDa): 0.013
Number of copies: 1

DNA-directed RNA polymerase II subunit RPB12 [Human]

Protein type
Name: DNA-directed RNA polymerase II subunit RPB12
Theoretical weight (MDa): 0.007
Number of copies: 1

Transcription initiation factor IIB [Homo sapiens]

Protein type
Name: Transcription initiation factor IIB
Theoretical weight (MDa): 0.035
Number of copies: 1
Expression system
Expression system: Escherichia coli

Transcription initiation factor IIA subunit 1 [Homo sapiens]

Protein type
Name: Transcription initiation factor IIA subunit 1
Theoretical weight (MDa): 0.042
Number of copies: 1
Expression system
Expression system: Escherichia coli

Transcription initiation factor IIA subunit 2 [Homo sapiens]

Protein type
Name: Transcription initiation factor IIA subunit 2
Theoretical weight (MDa): 0.012
Number of copies: 1
Expression system
Expression system: Escherichia coli

TATA-box-binding protein [Homo sapiens]

Protein type
Name: TATA-box-binding protein
Theoretical weight (MDa): 0.038
Number of copies: 1
Expression system
Expression system: Escherichia coli

General transcription factor IIE subunit 1 [Homo sapiens]

Protein type
Name: General transcription factor IIE subunit 1
Theoretical weight (MDa): 0.05
Number of copies: 1
Expression system
Expression system: Escherichia coli

Transcription initiation factor IIE subunit beta [Homo sapiens]

Protein type
Name: Transcription initiation factor IIE subunit beta
Theoretical weight (MDa): 0.033
Number of copies: 1
Expression system
Expression system: Escherichia coli

General transcription factor IIF subunit 1 [Homo sapiens]

Protein type
Name: General transcription factor IIF subunit 1
Theoretical weight (MDa): 0.058
Number of copies: 1
Expression system
Expression system: Escherichia coli

General transcription factor IIF subunit 2 [Homo sapiens]

Protein type
Name: General transcription factor IIF subunit 2
Theoretical weight (MDa): 0.028
Number of copies: 1
Expression system
Expression system: Escherichia coli

Transcription elongation factor A protein 1 [Homo sapiens]

Protein type
Name: Transcription elongation factor A protein 1
Theoretical weight (MDa): 0.034
Number of copies: 1
Expression system
Expression system: Escherichia coli

SCP-X [Homo sapiens]

Nucleic-acid type
Name: SCP-X
Theoretical weight (MDa): 0.023
Type: DNA
Structure: OTHER
Synthetic: false
Sequence: (DG)(DA)(DA)(DG)(DG)(DG)(DC)(DG)(DC)(DC)(DT)(DA)(DT)(DA)(DA)(DA)(DA)(DG)(DG)(DG) (DG)(DG)(DT)(DG)(DG)(DG)(DG)(DG)(DC)(DG)(DC)(DG)(DA)(DA)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DC)(DG)(DC)(DG)(DA)(DT)(DC)(DG)(DA)(DA)(DC)(DA)(DC)(DT)(DC)(DG) (DA)(DG)(DC)(DC)(DG)(DA)(DG)(DC)(DA)(DG)(DA)(DC)(DG)(DT)(DG)

SCP-Y [Homo sapiens]

Nucleic-acid type
Name: SCP-Y
Theoretical weight (MDa): 0.023
Type: DNA
Structure: OTHER
Synthetic: false
Sequence: (DC)(DA)(DC)(DG)(DT)(DC)(DT)(DG)(DC)(DT)(DC)(DG)(DG)(DC)(DT)(DC)(DG)(DA)(DG)(DT) (DG)(DT)(DT)(DC)(DG)(DA)(DT)(DC)(DG)(DC)(DG)(DA)(DC)(DT)(DG)(DA)(DG)(DG)(DA)(DC) (DG)(DA)(DA)(DC)(DG)(DC)(DG)(DC)(DC)(DC)(DC)(DC)(DA)(DC)(DC)(DC)(DC)(DC)(DT)(DT) (DT)(DT)(DA)(DT)(DA)(DG)(DG)(DC)(DG)(DC)(DC)(DC)(DT)(DT)(DC)

MAGNESIUM ION

Ligand type
Name: MAGNESIUM ION
Theoretical weight (MDa): 0
Number of copies: 2

ZINC ION

Ligand type
Name: ZINC ION
Theoretical weight (MDa): 0
Number of copies: 11