EMD-7922 Experiments and Validation

VIC133 Fab in complex with Ebola virus GP

Single particle reconstruction
Overview of EMD-7922
Sample name: Complex of VIC133 Fab bound to Ebola virus GP
Organism: Ebola virus - Mayinga, Zaire, 1976

Map parameters

Recommended contour level: 43.90 (author)
Number of grid points: 80 × 80 × 80
Voxel size: 4.10 × 4.10 × 4.10 Å
Minimum density: -45.639
Maximum density: 154.001
Average density: 1.069
Standard deviation: 10.09

Sample information

Sample name: Complex of VIC133 Fab bound to Ebola virus GP
Protein: Complex of VIC133 Fab bound to Ebola virus GP

Validation information

Experimental information

 

Image processing

Applied symmetry: C3
Number of particles: 22118

Imaging

Session
Microscope model: FEI TECNAI SPIRIT
Electron source: LAB6
Electron dose (e/A**2): 30.0
Nominal CS (mm): 2.20
Nominal magnification: 52000.0
Defocus max (nm): 1.00
Defocus min (nm): 1,500.00
Holder model: SIDE ENTRY, EUCENTRIC
Acquisition
Number of images: 96

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 0.02
Buffer: Solution was made from 10X concentration.
Staining procedure: Stained using 2% UF on carbon coated grids.
Vitrification
Cryogen: NONE