EMD-7780

Helical reconstruction
11.0 Å
EMD-7780
3D View Gallery
Deposition: 03/04/2018
Map released: 10/10/2018
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-7780
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6cxi summary report (pdf.gz) Map and 6cxi FULL report (pdf.gz)
EMICSS annotations (xml)

Cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C mode 1

EMD-7780

Helical reconstruction
11.0 Å
EMD-7780
3D View Gallery
Deposition: 03/04/2018
Map released: 10/10/2018
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Helical reconstruction
Aggregation State: Helical Array
Specimen preparation [1]
Buffer
pH: 7.0
Grid
Mesh: 300
Material: COPPER
Pretreatment
Type: PLASMA CLEANING
Time: {'units': 's', 'valueOf_': '15'}
Atmosphere: OTHER
Support Film [1]
Material Topology Thickness
CARBON LACEY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 95%
Chamber temperature: 294 K
Instrument: FEI VITROBOT MARK II
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Detector mode: INTEGRATING
Average electron dose per image: 20.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 11.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 6117
Algorithm: BACK PROJECTION
⌯ Applied Symmetry
ΔΖ: 27.5 Å
ΔΦ: -166.6°
Axial symmetry: C1
Software [1]
Name Version Details
SPIDER - IHRSR
Startup model [1]
Type: OTHER
Details:Cylinder density map
⦩ Final angle assignment
Type: NOT APPLICABLE
Software [1]
Name Version Details
SPIDER - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C
⬡ Geometry
X Y Z
Dimensions 230 230 230
Origin -115 -115 -114
Spacing 230 230 230
Voxel size 2.1 Å 2.1 Å 2.1 Å
Contour list
Primary Level Source
True 5304.0 AUTHOR