EMD-7620

Single-particle
3.3 Å
EMD-7620 Deposition: 25/03/2018
Map released: 16/05/2018
Last modified: 29/07/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-7620

Structure of the human TRPC3 in a lipid-occupied, closed state

EMD-7620

Single-particle
3.3 Å
EMD-7620 Deposition: 25/03/2018
Map released: 16/05/2018
Last modified: 29/07/2020
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Average electron dose per image: 6.76 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.3 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 143855
Applied Symmetry
Point group: C4
Software [1]
Name Version Details
FREALIGN 9 -
Initial angle assignment
Final angle assignment
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Structure of the human TRPC3 in a lipid-occupied, closed state
Geometry
X Y Z
Dimensions 384 384 384
Origin 0 0 0
Spacing 384 384 384
Voxel size 1.074 Å 1.074 Å 1.074 Å
Contour list
Primary Level Source
True 0.035 AUTHOR