EMD-7620
Structure of the human TRPC3 in a lipid-occupied, closed state
EMD-7620
Single-particle3.3 Å
Deposition: 25/03/2018
Map released: 16/05/2018
Last modified: 29/07/2020
Buffer
pH: 8.0
Vitrification
Cryogen name: ETHANE
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording
[1]
Detector model:
GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Average electron dose per image: 6.76 e/Å2
Detector mode: SUPER-RESOLUTION
Average electron dose per image: 6.76 e/Å2
Final
reconstruction
Resolution: 3.3
Å
(
BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 143855
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 143855
⌯ Applied Symmetry
Point group:
C4
Software
[1]
Name | Version | Details |
---|---|---|
FREALIGN | 9 | - |
⦨ Initial angle
assignment
Type:
ANGULAR RECONSTITUTION
⦩ Final angle assignment
Type:
ANGULAR RECONSTITUTION
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Structure of the human TRPC3 in a lipid-occupied, closed state
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Structure of the human TRPC3 in a lipid-occupied, closed state
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 384 | 384 | 384 |
Origin | 0 | 0 | 0 |
Spacing | 384 | 384 | 384 |
Voxel size | 1.074 Å | 1.074 Å | 1.074 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.035 | AUTHOR |