EMD-7574 Experiments and Validation

SARS Spike Glycoprotein, Stabilized variant, single upwards S1 CTD conformation

Single particle reconstruction
Overview of EMD-7574
Sample name: SARS spike glycoprotein, stabilized variant
Organisms: SARS coronavirus, Enterobacteria phage T4
Fitted atomic model: 6crw

Map parameters

Recommended contour level: 0.032 (author)
Number of grid points: 320 × 320 × 320
Voxel size: 1.03 × 1.03 × 1.03 Å
Minimum density: -0.082
Maximum density: 0.17
Average density: -0.00
Standard deviation: 0.006

Sample information

Sample name: SARS spike glycoprotein, stabilized variant
Ligands: N-ACETYL-D-GLUCOSAMINE, BETA-D-MANNOSE, ALPHA-D-MANNOSE
Proteins: SARS spike glycoprotein, stabilized variant, Spike glycoprotein,Fibritin

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Number of particles: 58349

Fitting

Initial atomic models: 5I08, 5I08, 5X4S, 2AJF
Used chains: A, A, A, E

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 65.0
Nominal CS (mm): 2.70
Nominal magnification: 29000.0
Defocus max (nm): 2,200.00
Defocus min (nm): 1,200.00
Holder model: FEI TITAN KRIOS AUTOGRID HOLDER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 0.35
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100