EMD-7501

Electron Crystallography
1.01 Å
EMD-7501
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Deposition: 02/03/2018
Map released: 16/05/2018
Last modified: 13/03/2024
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-7501
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
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EMICSS annotations (xml)

1.01 A MicroED structure of GSNQNNF at 0.17 e- / A^2

EMD-7501

Electron Crystallography
1.01 Å
EMD-7501
3D View Gallery
Deposition: 02/03/2018
Map released: 16/05/2018
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Electron Crystallography
Aggregation State: 3D Array
Specimen preparation [1]
Concentration: 10 mg/mL
Buffer
pH: 6.0
Buffer components [2]:
Name Formula Concentration ChEBI
MES C6H13NO4S 0.1 M
MPD C6H14O2 10.0 %
Grid
Mesh: 300
Model: Quantifoil R2/2
Material: COPPER
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Support Film [1]
Material Topology Thickness
CARBON HOLEY ARRAY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 30%
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: DIFFRACTION
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Camera Length: 730 mm
Specimen holder model: GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: TVIPS TEMCAM-F416 (4k x 4k)
Dimensions: 2048 pixel x 2048 pixel
Number of grids: 1
Number of real images: 1012
Number of diffraction images: 1012
Average exposure time: 2.1 s
Average electron dose per image: 0.00357 e/Å2
Image processing [1]
Final reconstruction
Resolution: 1.01 Å ( BY AUTHOR)
Resolution method: DIFFRACTION PATTERN/LAYERLINES
Crystallography statistics
Number of intensities measured: 14826
Number of structure factors: 1941
Fourier space coverage: 78.0
R sym: 0.224
R merge: 0.224
Overall phase error: 28.16
Overall phase residual: 28.16
Phase error rejection criteria: 0
High resolution: 1.01 Å
Shell list [1]
High resolution Low resolution Number of structure factors Phase residual Fourier space coverage Multiplicity
1.01 Å 1.04 Å 125 41.15 74.85 6.1
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: MicroED density map
⬡ Geometry
X Y Z
Dimensions 83 60 85
Origin -7 -36 23
Spacing 85 83 60
Voxel size 0.305 Å 0.32205 Å 0.33885 Å
Contour list
Primary Level Source
True 0.44538 AUTHOR