EMD-7498
1.15 A MicroED structure of GSNQNNF at 1.9 e- / A^2
EMD-7498
Electron Crystallography1.15 Å
Deposition: 02/03/2018
Map released: 16/05/2018
Last modified: 13/03/2024
Concentration: 10
mg/mL
Buffer
Grid
Mesh: 300
Model: Quantifoil R2/2
Material: COPPER
Model: Quantifoil R2/2
Material: COPPER
Pretreatment
Support Film [1]
Material | Topology | Thickness |
---|---|---|
CARBON | HOLEY ARRAY | - |
Vitrification
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: DIFFRACTION
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Camera Length: 730 mm
Specimen holder model: GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER
Cooling holder cryogen: NITROGEN
Illumination mode: FLOOD BEAM
Imaging mode: DIFFRACTION
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Camera Length: 730 mm
Specimen holder model: GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER
Cooling holder cryogen: NITROGEN
Image Recording
[1]
Detector model:
TVIPS TEMCAM-F416 (4k x 4k)
Dimensions: 2048 pixel x 2048 pixel
Number of grids: 1
Number of real images: 920
Number of diffraction images: 920
Average exposure time: 2.1 s
Average electron dose per image: 0.00588 e/Å2
Dimensions: 2048 pixel x 2048 pixel
Number of grids: 1
Number of real images: 920
Number of diffraction images: 920
Average exposure time: 2.1 s
Average electron dose per image: 0.00588 e/Å2
Final
reconstruction
Crystallography statistics
Number of intensities measured:
8705
Number of structure factors: 1337
Fourier space coverage: 81.2
R sym: 0.195
R merge: 0.195
Overall phase error: 37.91
Overall phase residual: 37.91
Phase error rejection criteria: 0
High resolution: 1.15 Å
Number of structure factors: 1337
Fourier space coverage: 81.2
R sym: 0.195
R merge: 0.195
Overall phase error: 37.91
Overall phase residual: 37.91
Phase error rejection criteria: 0
High resolution: 1.15 Å
Shell list
[1]
High resolution | Low resolution | Number of structure factors | Phase residual | Fourier space coverage | Multiplicity |
---|---|---|---|---|---|
1.15 Å | 1.18 Å | 117 | 16.26 | 84.17 | 6.0 |
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: MicroED density map
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: MicroED density map
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 70 | 53 | 74 |
Origin | -6 | -32 | 20 |
Spacing | 74 | 70 | 53 |
Voxel size | 0.34571 Å | 0.37211 Å | 0.4025 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 0.346905 | AUTHOR |