EMD-7444 Experiments and Validation

Electron cryo-microscopy of the eukaryotic translation initiation factor 2B from Homo sapiens

Single particle reconstruction
Overview of EMD-7444
Sample name: Translation initiation factor eIF-2B decamer
Organism: Homo sapiens
Related EM entry by publication: EMD-7442, EMD-7443

Map parameters

Recommended contour level: 0.008 (author)
Number of grid points: 364 × 364 × 364
Voxel size: 0.84 × 0.84 × 0.84 Å
Minimum density: -0.011
Maximum density: 0.028
Average density: 0.00
Standard deviation: 0.001

Sample information

Sample name: Translation initiation factor eIF-2B decamer
Protein: Translation initiation factor eIF-2B decamer

Validation information

Experimental information

 

Image processing

Applied symmetry: C2
Software: RELION
Number of particles: 202125

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 1.6299999999999999
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Vitrification
Apparatus: FEI VITROBOT MARK IV
Cryogen: ETHANE
Humidity: 100.0