EMD-6988

Helical reconstruction
3.07 Å
EMD-6988
3D View Gallery
Deposition: 27/06/2018
Map released: 11/07/2018
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-6988
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6a6b summary report (pdf.gz) Map and 6a6b FULL report (pdf.gz)
EMICSS annotations (xml)

cryo-em structure of alpha-synuclein fiber

EMD-6988

Helical reconstruction
3.07 Å
EMD-6988
3D View Gallery
Deposition: 27/06/2018
Map released: 11/07/2018
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Helical reconstruction
Aggregation State: Filament
Specimen preparation [1]
Buffer
pH: 7.5
Grid
Mesh: 300
Model: Quantifoil R1.2/1.3
Material: COPPER
Details: 4 microl of alpha-syn fibril solution was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3, 300 mesh), blotted for 6 s, and plunge-frozen in liquid ethane using FEI Vitrobot Mark IV. 95% humidity, 16 degrees
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Vitrification
Cryogen name: ETHANE
Chamber humidity: 95%
Chamber temperature: 289 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Frames per image: 1-32
Average exposure time: 8.0 s
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.07 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 43677
⌯ Applied Symmetry
ΔΖ: 2.393 Å
ΔΦ: 179.641°
Axial symmetry: C1
Software [1]
Name Version Details
RELION 2.1 -
Startup model [1]
Type: OTHER
Details:A feature less cylinder
⦩ Final angle assignment
Type: NOT APPLICABLE
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Density map calculated by applying a helical symmetry to the segments in the middle of 3D reconstruction. The twist angle is 179.64 degree and the rise is 3.39 angstrom.
⬡ Geometry
X Y Z
Dimensions 200 200 200
Origin 0 0 0
Spacing 200 200 200
Voxel size 1.32 Å 1.32 Å 1.32 Å
Contour list
Primary Level Source
True 0.02 AUTHOR