EMD-6799

Electron Crystallography
6.5 Å
EMD-6799
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Deposition: 16/07/2017
Map released: 09/08/2017
Last modified: 09/08/2017
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-6799
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5y0b summary report (pdf.gz) Map and 5y0b FULL report (pdf.gz)
EMICSS annotations (xml)

PIG GASTRIC H+,K+ - ATPASE IN COMPLEX with BYK99

EMD-6799

Electron Crystallography
6.5 Å
EMD-6799
3D View Gallery
Deposition: 16/07/2017
Map released: 09/08/2017
Last modified: 09/08/2017
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Electron Crystallography
Aggregation State: 2D Array
Specimen preparation [1]
Concentration: 6.5 mg/mL
Details: This sample is 2D crystal.
Buffer
pH: 4.8
Vitrification
Cryogen name: NITROGEN
Instrument: LEICA KF80
Microscopy [1]
Microscope: JEOL KYOTO-3000SFF
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Camera Length: 1500 mm
Image Recording [1]
Detector model: KODAK SO-163 FILM
Average electron dose per image: 8.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 6.5 Å ( BY AUTHOR)
Resolution method: DIFFRACTION PATTERN/LAYERLINES
Software [1]
Name Version Details
MRC - -
Crystal parameters
A: 142.6 Å
B: 112.0 Å
C: 320 Å
C sampling length: 320 Å
γ: 90°
Plane group: P 2 21 21
CTF correction
Software [1]
Name Version Details
MRC - -
Crystallography statistics
Number of intensities measured: 59212
Number of structure factors: 4974
Fourier space coverage: 47.1
R sym: 0.469
R merge: 0.469
Overall phase error: 33.1
Overall phase residual: 33.1
Phase error rejection criteria: 0.0
High resolution: 6.5 Å
Shell list [1]
High resolution Low resolution Number of structure factors Phase residual Fourier space coverage Multiplicity
6.5 Å 200.0 Å 4974 33.1 47.1 11.9
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 289 137 105
Origin -144 -68 -52
Spacing 136 104 288
Voxel size 1.0485 Å 1.0769 Å 1.1111 Å
Contour list
Primary Level Source
True 0.004 AUTHOR