EMD-6744 Experiments and Validation

Structure of M-LHCII and CP24 complexes in the unstacked C2S2M2-type PSII-LHCII supercomplex from Pisum sativum

Single particle reconstruction
Overview of EMD-6744
Sample name: M-LHCII and CP24 complexes in the unstacked C2S2M2-type PSII-LHCII supercomplex from Pisum sativum
Organisms: Pisum sativum, Garden pea
Fitted atomic model: 5xno
Related EM entries by publication: EMD-6741, EMD-6742, EMD-6743

Map parameters

Recommended contour level: 0.028 (author)
Number of grid points: 150 × 150 × 150
Voxel size: 1.04 × 1.04 × 1.04 Å
Minimum density: -0.039
Maximum density: 0.088
Average density: 0.001
Standard deviation: 0.007

Sample information

Sample name: M-LHCII and CP24 complexes in the unstacked C2S2M2-type PSII-LHCII supercomplex from Pisum sativum
Ligands: CHLOROPHYLL B, CHLOROPHYLL A, (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL, (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL, (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL, 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE, BETA-CAROTENE
Proteins: M-LHCII and CP24 complexes in the unstacked C2S2M2-type PSII-LHCII supercomplex from Pisum sativum, Chlorophyll a-b binding protein 8, chloroplastic, Chlorophyll a-b binding protein, chloroplastic, Light harvesting chlorophyll a/b-binding protein Lhcb6, CP24

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Number of particles: 50237

Fitting

Refinement space: REAL

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 60.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 3.00
Vitrification
Cryogen: ETHANE