EMD-6566

Single-particle
3.46 Å
EMD-6566
3D View Gallery
Deposition: 23/12/2015
Map released: 10/02/2016
Last modified: 10/02/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-6566
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 3jcm summary report (pdf.gz) Map and 3jcm FULL report (pdf.gz)
EMICSS annotations (xml)

The cryo-EM structure of yeast spliceosomal U4/U6.U5 tri-snRNP (improved map for Prp3 region at 3.46 A)

EMD-6566

Single-particle
3.46 Å
EMD-6566
3D View Gallery
Deposition: 23/12/2015
Map released: 10/02/2016
Last modified: 10/02/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Vitrification
Cryogen name: ETHANE
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image Recording [1]
Detector category: FILM
Detector model: GATAN K2 SUMMIT (4k x 4k)
Scanner: TEMSCAN
Number of real images: 3141
Image processing [1]
Final reconstruction
Resolution: 3.46 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 172134
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of tri-snRNP
Details: ::::EMDATABANK.org::::EMD-6566::::
⬡ Geometry
X Y Z
Dimensions 320 320 320
Origin 0 0 0
Spacing 320 320 320
Voxel size 1.32 Å 1.32 Å 1.32 Å
Contour list
Primary Level Source
True 0.0233 AUTHOR