EMD-6416

Single-particle
3.3 Å
EMD-6416
3D View Gallery
Deposition: 09/08/2015
Map released: 16/09/2015
Last modified: 08/06/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-6416
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 3jb9 summary report (pdf.gz) Map and 3jb9 FULL report (pdf.gz)
EMICSS annotations (xml)

Local map for Cwf10/Sm region of the yeast spliceosome at 3.30 angstrom resolution

EMD-6416

Single-particle
3.3 Å
EMD-6416
3D View Gallery
Deposition: 09/08/2015
Map released: 16/09/2015
Last modified: 08/06/2016
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: SPOT SCAN
Imaging mode: DARK FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image Recording [1]
Detector category: FILM
Detector model: GATAN K2 (4k x 4k)
Scanner: TEMSCAN
Number of real images: 2246
Image processing [1]
Final reconstruction
Resolution: 3.3 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 112795
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION - -
CTF correction
Details:Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction by applying local mask for Cwf10/Snu114 and Sm
Details: ::::EMDATABANK.org::::EMD-6416::::
⬡ Geometry
X Y Z
Dimensions 360 360 360
Origin 0 0 0
Spacing 360 360 360
Voxel size 1.32 Å 1.32 Å 1.32 Å
Contour list
Primary Level Source
True 0.0139 AUTHOR