EMD-6289

Single-particle
6.5 Å
EMD-6289
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Deposition: 27/02/2015
Map released: 11/03/2015
Last modified: 27/05/2015
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-6289
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Three dimensional reconstruction of bovine dynactin complex by cryo electron microscopy

EMD-6289

Single-particle
6.5 Å
EMD-6289
3D View Gallery
Deposition: 27/02/2015
Map released: 11/03/2015
Last modified: 27/05/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: Three-dimensional reconstruction of bovine dynactin complex by cryo EM
Sample [1]
Name: Three-dimensional reconstruction of bovine dynactin complex by cryo EM
Number of unique components: 8
Oligomeric state: One CapZ alpha, one capZ beta, eight Arp1, one actin, one Arp11, one p25, one p27, one p62
Details: The sample was monodisperse. The shoulder domain of dynactin would dissociate during cryo grid preparation.
Molecular weight
Experimental Theoretical Method
- 1 MDa -
Protein [8]
Name: Arp1
Number of copies: 8
Oligomeric state: Octamer
Natural source
Organism: Bos taurus (bovine)
Tissue: Brain
Cell: Neuron
Cellular location: Cytoplasm
Molecular weight
Experimental Theoretical Method
- 42 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
- - - -
Name: Actin
Number of copies: 1
Oligomeric state: Monomer
Natural source
Organism: Bos taurus (bovine)
Tissue: Brain
Cell: Neuron
Cellular location: Cytoplasm
Molecular weight
Experimental Theoretical Method
- 42 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
- - - -
Name: Arp11
Number of copies: 1
Oligomeric state: Monomer
Natural source
Organism: Bos taurus (bovine)
Tissue: Brain
Cell: Neuron
Cellular location: Cytoplasm
Molecular weight
Experimental Theoretical Method
- 42 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
- - - -
Name: p62
Number of copies: 1
Oligomeric state: Monomer
Natural source
Organism: Bos taurus (bovine)
Tissue: Brain
Cell: Neuron
Cellular location: Cytoplasm
Molecular weight
Experimental Theoretical Method
- 62 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
- - - -
Name: p25
Number of copies: 1
Oligomeric state: Monomer
Natural source
Organism: Bos taurus (bovine)
Tissue: Brain
Cell: Neuron
Cellular location: Cytoplasm
Molecular weight
Experimental Theoretical Method
- 25 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
- - - -
Name: p27
Number of copies: 1
Oligomeric state: Monomer
Natural source
Organism: Bos taurus (bovine)
Tissue: Brain
Cell: Neuron
Cellular location: Cytoplasm
Molecular weight
Experimental Theoretical Method
- 27 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
- - - -
Name: CapZ alpha
Number of copies: 1
Oligomeric state: Monomer
Natural source
Organism: Bos taurus (bovine)
Tissue: Brain
Cell: Neuron
Cellular location: Cytoplasm
Molecular weight
Experimental Theoretical Method
- 33 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
- - - -
Name: CapZ beta
Number of copies: 1
Oligomeric state: Monomer
Natural source
Organism: Bos taurus (bovine)
Tissue: Brain
Cell: Neuron
Cellular location: Cytoplasm
Molecular weight
Experimental Theoretical Method
- 33 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
- - - -