EMD-6255

Single-particle
23.4 Å
EMD-6255
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Deposition: 23/01/2015
Map released: 18/02/2015
Last modified: 27/05/2015
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-6255
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Negative stain reconstruction of the Pex1/Pex6 complex in presence of ATP

EMD-6255

Single-particle
23.4 Å
EMD-6255
3D View Gallery
Deposition: 23/01/2015
Map released: 18/02/2015
Last modified: 27/05/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Name: Pex1/Pex6 complex in presence of 3 mM ATP
Sample [1]
Name: Pex1/Pex6 complex in presence of 3 mM ATP
Number of unique components: 2
Oligomeric state: Heterohexamer containing three copies of Pex1 and three copies of Pex6
Details: 22 nM Pex1/Pex6 complex in presence of 3 mM ATP was used for negative stain electron microscopy. The sample was monodisperse.
Molecular weight
Experimental Theoretical Method
- 707 kDa -
Protein [2]
Recombinant expression
Name: Peroxisomal ATPase PEX1 (Peroxin-1, Peroxisomal assembly protein 1, Peroxisome biogenesis protein PAS1)
Number of copies: 3
Oligomeric state: Heterohexamer
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
peroxisome organization protein targeting to peroxisome protein import into peroxisome matrix, receptor recycling
Molecular function:
ATP hydrolysis activity ATP hydrolysis activity nucleotide binding protein heterodimerization activity protein binding ATP binding
Cellular location:
membrane peroxisome peroxisomal membrane cytoplasm
Natural source
Organism: Saccharomyces cerevisiae (Baker's yeast)
Organelle: peroxisome, cytosol
Cellular location: peroxisome membrane
Molecular weight
Experimental Theoretical Method
117 kDa 117 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - pCOLA-DUET
Recombinant expression
Name: Peroxisomal ATPase PEX6 (Peroxin-6 Peroxisomal assembly protein 8, Peroxisome biosynthesis protein PAS8)
Number of copies: 3
Oligomeric state: Heterohexamer
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [13]
Biological process:
protein import into peroxisome matrix, receptor recycling obsolete replicative cell aging peroxisome organization
Molecular function:
ATP binding nucleotide binding ATP hydrolysis activity protein binding protein heterodimerization activity
Cellular location:
cytoplasm cytosol peroxisomal membrane membrane peroxisome
Natural source
Organism: Saccharomyces cerevisiae (Baker's yeast)
Organelle: peroxisome, cytosol
Cellular location: peroxisome membrane
Molecular weight
Experimental Theoretical Method
115 kDa 115 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - pET-DUET