EMD-5897
Cryo EM density of microtubules stabilized by taxol
EMD-5897
Single-particle5.5 Å
Deposition: 09/02/2014
Map released: 04/06/2014
Last modified: 04/06/2014
Concentration: 0.25
mg/mL
Buffer
pH: 6.8
Details: 80mM PIPES, 1mM EGTA, 1mM MgCl2, 1mM DTT, 0.05% Nonidet P-40
Details: 80mM PIPES, 1mM EGTA, 1mM MgCl2, 1mM DTT, 0.05% Nonidet P-40
Grid
Details: 400 mesh C-flat 1.2/1.3, glow discharged in Edwards carbon evaporator
Vitrification
Cryogen name: ETHANE
Chamber humidity: 90%
Chamber temperature: 90.4 K
Instrument: FEI VITROBOT MARK II
Method: The grid was blotted for 2 seconds before plunging.
Chamber humidity: 90%
Chamber temperature: 90.4 K
Instrument: FEI VITROBOT MARK II
Method: The grid was blotted for 2 seconds before plunging.
Microscope: FEI TITAN
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.4 µm - 3.5 µm
Nominal magnification: 72000.0
Calibrated magnification: 72000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Gatan 626 holder
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 100,000 times magnifacation., Electron beam tilt params: )
Minimum tilt angle: 0
Maximum tilt angle: 0
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.4 µm - 3.5 µm
Nominal magnification: 72000.0
Calibrated magnification: 72000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Gatan 626 holder
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 100,000 times magnifacation., Electron beam tilt params: )
Minimum tilt angle: 0
Maximum tilt angle: 0
Image Recording
[1]
Detector category:
FILM
Detector model: KODAK SO-163 FILM
Scanner: OTHER
Sampling interval: 6.35 µm
Number of real images: 149
Average electron dose per image: 25.0 e/Å2
Bits per pixel: 16.0
Detector model: KODAK SO-163 FILM
Scanner: OTHER
Sampling interval: 6.35 µm
Number of real images: 149
Average electron dose per image: 25.0 e/Å2
Bits per pixel: 16.0
Details: Initial alignments performed with EMAN2/SPARX, including refinement of helical parameters with the IHRSR programs of Egelman. Final alignment and reconstruction performed with FREALIGN.
Final
reconstruction
CTF correction
Details:Ctftilt
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of microtubule stabilized by taxol
Details: ::::EMDATABANK.org::::EMD-5897::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM reconstruction of microtubule stabilized by taxol
Details: ::::EMDATABANK.org::::EMD-5897::::
⬡ Geometry
X | Y | Z | |
---|---|---|---|
Dimensions | 110 | 66 | 162 |
Origin | -55 | -33 | -81 |
Spacing | 66 | 110 | 162 |
Voxel size | 1.7399999 Å | 1.74 Å | 1.74 Å |
Contour list
Primary | Level | Source |
---|---|---|
True | 4.0 | EMDB |