EMD-5426

Single-particle
9.3 Å
EMD-5426
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Deposition: 29/05/2012
Map released: 04/06/2012
Last modified: 04/06/2012
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EMD-5426
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Cryo-EM reconstruction of Nudaurelia capensis omega virus (NwV) capsid at 3min time point following maturation initiation

EMD-5426

Single-particle
9.3 Å
EMD-5426
3D View Gallery
Deposition: 29/05/2012
Map released: 04/06/2012
Last modified: 04/06/2012
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 10 mg/mL
Buffer
pH: 5.0
Details: 100mM NaOAc, 250mM NaCl
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 90 K
Instrument: FEI VITROBOT MARK I
Method: Blot for 4sec before plunging
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal defocus: 1.0 µm - 3.0 µm
Nominal magnification: 80000.0
Specimen holder model: GATAN LIQUID NITROGEN
Image Recording [1]
Number of real images: 102
Average electron dose per image: 16.5 e/Å2
Details: Data were collected using the Leginon automated electron microscopy package and data processing was performed using the Appion pipeline.
Image processing [1]
Final reconstruction
Resolution: 9.3 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 6087
Algorithm: OTHER
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
EMAN - -
CTF correction
Details:Each image
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of Nudaurelia capensis omega virus (NwV) capsid at 3min time point following maturation initiation
Details: ::::EMDATABANK.org::::EMD-5426::::
⬡ Geometry
X Y Z
Dimensions 200 200 200
Origin -100 -100 -100
Spacing 200 200 200
Voxel size 2.768 Å 2.768 Å 2.768 Å
Contour list
Primary Level Source
True 1.0 AUTHOR