EMD-4859

Single-particle
4.2 Å
EMD-4859
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Deposition: 12/04/2019
Map released: 24/04/2019
Last modified: 06/11/2019
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EMD-4859
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Cryo-EM structure of the anti-feeding prophage (AFP) sheath-tube in contracted state, C6 symmetrized

EMD-4859

Single-particle
4.2 Å
EMD-4859
3D View Gallery
Deposition: 12/04/2019
Map released: 24/04/2019
Last modified: 06/11/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Filament
Specimen preparation [1]
Buffer
pH: 7.0
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Average electron dose per image: 27.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.2 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 15189
⌯ Applied Symmetry
Point group: C6
Software [1]
Name Version Details
RELION - -
Startup model [1]
Type: OTHER
Details:Previous 20A map of AFP (Heymann et al., 2013)
⦨ Initial angle assignment
Type: PROJECTION MATCHING
⦩ Final angle assignment
Type: PROJECTION MATCHING
Particle selection [1]
Selected Ref. model Method Software Details
15189 - - - -
CTF correction
Software [1]
Name Version Details
CTFFIND - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: None
⬡ Geometry
X Y Z
Dimensions 364 364 364
Origin -182 -182 -182
Spacing 364 364 364
Voxel size 1.397 Å 1.397 Å 1.397 Å
Contour list
Primary Level Source
True 0.12 AUTHOR