EMD-4785 Experiments and Validation

D. melanogaster CMG-DNA with ATP, State 1A

Single particle reconstruction
Overview of EMD-4785
Sample name: CMG helicase bound to forked DNA in the presence of ATP, State 1A
Organism: Drosophila melanogaster
Fitted atomic model: 6raw
Related EM entries by publication: EMD-4786, EMD-4787, EMD-4788

Map parameters

Recommended contour level: 0.007 (author)
Number of grid points: 384 × 384 × 384
Voxel size: 1.08 × 1.08 × 1.08 Å
Minimum density: -0.031
Maximum density: 0.047
Average density: 0.00
Standard deviation: 0.001

Sample information

Sample name: CMG helicase bound to forked DNA in the presence of ATP, State 1A
Ligands: ADENOSINE-5'-TRIPHOSPHATE, ADENOSINE-5'-DIPHOSPHATE
Nucleic acids: DNA, DNA (5'-D(P*TP*CP*GP*AP*TP*CP*GP*AP*TP*CP*GP*AP*T)-3')
Proteins: CMG helicase bound to forked DNA in the presence of ATP, State 1A, CMG helicase, DNA, DNA replication licensing factor Mcm2, DNA replication licensing factor Mcm3, DNA replication licensing factor MCM4, DNA replication licensing factor Mcm5, DNA replication licensing factor Mcm6, DNA replication licensing factor Mcm7, CDC45L, IP07275p, Probable DNA replication complex GINS protein PSF2, AT18545p, DNA replication complex GINS protein SLD5

Validation information

Experimental information

 

Image processing

Number of particles: 170329

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 50.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Vitrification
Cryogen: ETHANE