EMD-4683

Single-particle
4.2 Å
EMD-4683
3D View Gallery
Deposition: 10/03/2019
Map released: 12/06/2019
Last modified: 06/11/2019
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Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-4683
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The cryo-EM structure of the tail knob in genome empited bacteriophage phi29

EMD-4683

Single-particle
4.2 Å
EMD-4683
3D View Gallery
Deposition: 10/03/2019
Map released: 12/06/2019
Last modified: 06/11/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.5
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 40.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.2 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 31478
⦨ Initial angle assignment
Type: PROJECTION MATCHING
⦩ Final angle assignment
Type: PROJECTION MATCHING
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 540 540 540
Origin -268 -268 -268
Spacing 540 540 540
Voxel size 1.36 Å 1.36 Å 1.36 Å
Contour list
Primary Level Source
True 1.0 AUTHOR