EMD-4552

Single-particle
3.75 Å
EMD-4552
3D View Gallery
Deposition: 18/01/2019
Map released: 10/04/2019
Last modified: 18/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-4552
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6qi8 summary report (pdf.gz) Map and 6qi8 FULL report (pdf.gz)
EMICSS annotations (xml)

Truncated human R2TP complex, structure 3 (ADP-filled)

EMD-4552

Single-particle
3.75 Å
EMD-4552
3D View Gallery
Deposition: 18/01/2019
Map released: 10/04/2019
Last modified: 18/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.8
Grid
Model: Quantifoil R1.2/1.3
Material: COPPER
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 1.18 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.75 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 102100
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 3.0 -
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Particle selection [1]
Selected Ref. model Method Software Details
408781 - - - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Dimensions 260 260 260
Origin 0 0 0
Spacing 260 260 260
Voxel size 1.09 Å 1.09 Å 1.09 Å
Contour list
Primary Level Source
True 0.02 AUTHOR