Electron Microscopy Data Bank

EMD-4550

Single-particle
7.3 Å

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Deposition: 17/01/2019
Map released: 12/06/2019
Last modified: 26/06/2019

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EMD-4550

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Cryo-EM structure of human norovirus GII.4 NSW-2012 VLP with T=4 icosahedral symmetry

EMD-4550

Single-particle
7.3 Å
EMD-4550

3D View Gallery

Deposition: 17/01/2019
Map released: 12/06/2019
Last modified: 26/06/2019

Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

Method: Single-particle
Aggregation State: Particle

Summary:

Specimen preparation
Microscopy
Image processing
Map

Specimen preparation [1]

Concentration: 4 mg/mL

Buffer

pH: 7.4
Buffer components [1]:

Name	Formula	Concentration	ChEBI
PBS	-	-	-

Grid

Mesh: 300
Model: Quantifoil R1.2/1.3
Material: COPPER/RHODIUM

Vitrification

Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV

Microscopy [1]

Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal magnification: 64000.0

Image Recording [1]

Detector model: GATAN K2 SUMMIT (4k x 4k)
Number of grids: 1
Number of real images: 364
Average electron dose per image: 20.0 e/Å²

Image processing [1]

Final reconstruction

Resolution: 7.3 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 10548

Applied Symmetry

Point group: I

Software [1]

Name	Version	Details
RELION	2.1	-

Initial angle assignment

Type: MAXIMUM LIKELIHOOD

Final angle assignment

Type: MAXIMUM LIKELIHOOD

Map

Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM structure of GII.4 NSW virus like particle

Geometry

	X	Y	Z
Dimensions	256	256	256
Origin	0	0	0
Spacing	256	256	256
Voxel size	2.27 Å	2.27 Å	2.27 Å

Contour list

Primary	Level	Source
True	0.05	AUTHOR