EMD-4529

Single-particle
5.9 Å
EMD-4529
3D View Gallery
Deposition: 03/01/2019
Map released: 15/05/2019
Last modified: 15/05/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-4529
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3D volume (map.gz) Experimental metadata (xml)
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EMICSS annotations (xml)

Human post-catalytic spliceosome (P complex), focused refinement on Aquarius and SYF1

EMD-4529

Single-particle
5.9 Å
EMD-4529
3D View Gallery
Deposition: 03/01/2019
Map released: 15/05/2019
Last modified: 15/05/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 1 mg/mL
Buffer
pH: 7.9
Buffer components [3]:
Name Formula Concentration ChEBI
HEPES - 20.0 mM -
- KCl 100.0 mM
EDTA - 0.2 mM -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK III
Details: 3 uL sample was applied to the grid, left for 25s, then blotted for 2.5-3.5s and immediately plunged into liquid ethane..
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Nominal defocus: 1.5 µm - 3.5 µm
Nominal magnification: 130000.0
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 60.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 5.9 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 106663
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
RELION 3.0 -
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 3.0 -
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Software [1]
Name Version Details
RELION 3.0 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Human post-catalytic spliceosome (P complex), focused refinement on Aquarius and SYF1
⬡ Geometry
X Y Z
Dimensions 410 410 410
Origin 0 0 0
Spacing 410 410 410
Voxel size 1.2 Å 1.2 Å 1.2 Å
Contour list
Primary Level Source
True 0.018 AUTHOR