EMD-4467 Experiments and Validation

Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2delD1-Cks2 complex (with the D1 box mutated)

Single particle reconstruction
Overview of EMD-4467
Sample name: APC/C-Cdc20-Cdk2-cyclinA2delD1-Cks2 complex (with the D1 box mutated)
Organism: Homo sapiens
Related EM entries by publication: EMD-4463, EMD-4464, EMD-4465, EMD-4466

Map parameters

Recommended contour level: 0.014 (author)
Number of grid points: 400 × 400 × 400
Voxel size: 1.10 × 1.10 × 1.10 Å
Minimum density: -0.031
Maximum density: 0.069
Average density: 0.00
Standard deviation: 0.002

Sample information

Sample name: APC/C-Cdc20-Cdk2-cyclinA2delD1-Cks2 complex (with the D1 box mutated)
Proteins: APC/C-Cdc20-Cdk2-cyclinA2delD1-Cks2 complex (with the D1 box mutated), Apc1, Apc2, Apc3, Apc4, Apc5, Apc6, Apc7, Apc8, Apc10, Apc11, Apc12, Apc13, Apc15, Apc16, Cdc20, Cdk2, Cyclin A2, Cks2

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Number of particles: 61415

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 28.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 0.15
Buffer: 20mM Hepes, 150mM NaCl, 0.5mM TCEP
Vitrification
Cryogen: ETHANE