EMD-4466 Experiments and Validation

Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2-Cks2 complex, the D2 box class

Single particle reconstruction
Overview of EMD-4466
Sample name: Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2-Cks2 complex, the D2 box class
Organism: Homo sapiens
Fitted atomic model: 6q6h
Related EM entries by publication: EMD-4463, EMD-4464, EMD-4465, EMD-4467

Map parameters

Recommended contour level: 0.008 (author)
Number of grid points: 400 × 400 × 400
Voxel size: 1.05 × 1.05 × 1.05 Å
Minimum density: -0.034
Maximum density: 0.057
Average density: 0.00
Standard deviation: 0.004

Sample information

Sample name: Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2-Cks2 complex, the D2 box class
Proteins: Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2-Cks2 complex, the D2 box class, Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2-Cks2 complex, the D2 box class, Cryo-EM structure of the APC/C-Cdc20-Cdk2-cyclinA2-Cks2 complex, the D2 box class, Anaphase-promoting complex subunit 10, Anaphase-promoting complex subunit 15, Apc1, Anaphase-promoting complex subunit 2, Anaphase-promoting complex subunit 4, Anaphase-promoting complex subunit 5, Cell division cycle protein 16 homolog, Anaphase-promoting complex subunit 11, Anaphase-promoting complex subunit CDC26, Anaphase-promoting complex subunit 13, Anaphase-promoting complex subunit 16, Cell division cycle protein 27 homolog, Anaphase-promoting complex subunit 7, Cell division cycle protein 23 homolog, Cell division cycle protein 20 homolog, Cyclin-A2

Validation information

Experimental information

 

Image processing

Applied symmetry: C1
Software: RELION
Number of particles: 117044

Imaging

Session
Microscope model: FEI TITAN KRIOS
Electron source: FIELD EMISSION GUN
Electron dose (e/A**2): 28.0
Holder model: OTHER

Specimen

Preparation
Specimen state: particle
Specimen concentration (mg/mL): 0.15
Buffer: 20mM Hepes, 150mM NaCl, 0.5mM TCEP
Vitrification
Cryogen: ETHANE